Journal: BMC Genomics

Article Title: Identification of novel vascular markers through gene expression profiling of tumor-derived endothelium

doi: 10.1186/1471-2164-9-201

Figure Lengend Snippet: Transcriptional differences: tumor vs normal tissue derived EC

Article Snippet: In situ hybridization experiments were carried out with a mixture of specific biotin-labeled oligonucleotide antisense (or sense) probes for ADAM23, GPNMB and PRSS3 (Metabion International AG, Martinsried, Germany) listed in the supplementary Table 2 [see Additional file ].

Techniques: Derivative Assay, Membrane, Immunopeptidomics, Activation Assay, Binding Assay, Variant Assay, Dominant Negative Mutation, Wilms Tumor Assay

Journal: BMC Genomics

Article Title: Identification of novel vascular markers through gene expression profiling of tumor-derived endothelium

doi: 10.1186/1471-2164-9-201

Figure Lengend Snippet: Gene expression in different cell lines . Levels of expression of ADAM23, FAP, GPNMB and PRSS3 were evaluated in fibroblasts (HuFb and Malme3) and smooth muscle cells (USMAC) as well as in tumor cell lines (1A9, SKOV3, HT29 and MDA-MB-231). Gene expression of each target gene was normalized to 18s rRNA for each cell type (ΔCt = Ct target - Ct 18s ). Average of ΔCt of the target gene from tumor derived ECs (Figure 1) was assumed as reference (ΔCt ref ). Fold differences for each cell type were calculated according to the comparative ΔΔCt method (Fold difference = 2 -(ΔCt each celltype-ΔCt ref) ) and expressed as percentage relative to tumor-derived EC (100%).

Article Snippet: In situ hybridization experiments were carried out with a mixture of specific biotin-labeled oligonucleotide antisense (or sense) probes for ADAM23, GPNMB and PRSS3 (Metabion International AG, Martinsried, Germany) listed in the supplementary Table 2 [see Additional file ].

Techniques: Gene Expression, Expressing, Derivative Assay

Journal: BMC Genomics

Article Title: Identification of novel vascular markers through gene expression profiling of tumor-derived endothelium

doi: 10.1186/1471-2164-9-201

Figure Lengend Snippet: In situ hybridization of human tissues . Shown are representative samples of medulloblastoma (A), brain metastasis of an adenocarcinoma (B) and Ewing sarcoma (C), hybridized with antisense probes for ADAM23, GPNMB and PRSS3, respectively. Positively stained blood vessels are indicated by arrows. Related hybridization with control sense probes for ADAM23, GPNMB and PRSS3 is shown in panel D-E-F. Normal brain samples, from different donors, hybridized respectively with antisense probes for ADAM23 (G) and GPNMB (H) are shown. Alzheimer's-diseased brain hybridized with antisense probe for PRSS3 is shown (I).

Article Snippet: In situ hybridization experiments were carried out with a mixture of specific biotin-labeled oligonucleotide antisense (or sense) probes for ADAM23, GPNMB and PRSS3 (Metabion International AG, Martinsried, Germany) listed in the supplementary Table 2 [see Additional file ].

Techniques: In Situ Hybridization, Staining, Hybridization, Control